female human coronary artery vsmcs  (Thermo Fisher)

Journal: American Journal of Physiology - Cell Physiology

Article Title: Palmitate induces glycosylation of cyclooxygenase-2 in primary human vascular smooth muscle cells

doi: 10.1152/ajpcell.00254.2017

Figure Lengend Snippet: Table 1.

Article Snippet: Primary adult male human brain vascular smooth muscle cells (HBVSM) (lot no. ACBRI 405, Cell Systems) and female human coronary vascular smooth muscle cells (HCVSM) (lot no. 0000289727, Lonza) were also grown under the same conditions as the HAoVSM and were included in these studies 1 ) to assess palmitate’s effect on COX-2 expression in cells from another vascular bed origin and 2 ) to investigate primary vascular cells from additional donors.

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Journal:

Article Title: Resveratrol, a Red Wine Constituent, Blocks the Antimitogenic Effects of Estradiol on Human Female Coronary Artery Smooth Muscle Cells

doi: 10.1210/jc.2010-0460

Figure Lengend Snippet: Expression of ER-α and ER-β in coronary artery smooth muscle cells (A) and attenuation by resveratrol (Resv; 5 μmol/liter) of the concentration-dependent inhibitory effects of estradiol (1–100 nmol/liter) on 2.5% serum (FCS)-induced DNA synthesis (B), [3H]proline incorporation (C), and cell proliferation (D) in human VSMCs. Values represent mean ± sem from at least four independent experiments, each conducted in triplicate. *, P < 0.05 vs. control cells treated with FCS alone; §, significant reversal of the inhibitory effects of estradiol.

Article Snippet: Cell culture Female human coronary artery VSMCs were obtained from Cascade Biologics (Portland, OR) and cultured as described previously ( 13 ) .

Techniques: Expressing, Concentration Assay, DNA Synthesis